Comparison of Urinary Bladder, Heart, Spleen, and Ear Biopsy Samples for Determining Prevalence of Borrelia burgdorferi in the white-footed deer mouse (Peromyscus Ieucopus) using four different primers

By Kacie Chern

Polymerase Chain Reaction (PCR) was used to compare urinary bladder, heart, spleen and ear biopsy samples from white-footed mice for the presence of Borrelia burgdorferi sensu lato.

Urinary bladder samples led to a significantly higher estimate of infection.

This suggests that the use of different tissue types in epidemiological studies of B. burgdorferi in an endemic site is likely to lead to underestimates of the prevalence.

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