Agilent LC/Q-TOF Mass spec
Liquid chromatography (LC) separates the sample components and then introduces them to the mass spectrometer (MS). The MS creates and detects charged ions. Once detected, spectra of relative abundance against the ratio of mass/charge (m/z) are generated. These spectra are used to determine molecular masses of molecules under investigation, provide more data for structural elucidation, detect reaction (by) products.
Agilent 6545XT AdvanceBio MS QTOF is coupled with the 1260 Infinity II LC system This LC offers a broad range of analytical applications with high reliability, performance, and precision. The QTOF is designed to optimize the characterization of biomolecules. It possesses the SWARM autotune function for optimal performance and detection at the sub-nanogram level with mass accuracies within 10 ppm. Its application in proteomics includes peptide mapping, detection of PTMs, intact protein analysis, etc. It is also suitable for metabolomics, forensic analyses, environmental analyses, and clinical applications.
Agilent Gas Chromatography Mass Spectrometry (GC/MS)
The Agilent GC-MS targets small and volatile molecules. It has high performance for a broad range of samples and applications. It is the analysis method of choice for smaller and volatile molecules such as alcohols, aromatics, and simple molecules such as steroids, fatty acids, and hormones. It can be used for the analyses of lipids, hormones, and fatty acid content in foods (Agro industry), environmental contaminants, and blood toxins (toxicology/forensics).
This instrument has a high retention time and area reproducibility due to its unique temperature and pneumatic control. Its digital range supports the quantification of small and larger sample peaks in one run. This instrument has standard Atmospheric pressure and temperature balance; hence fluctuations in the lab environment do not impact results. This instrument has a high retention time and area reproducibility due to its unique temperature and pneumatic control. Its digital range supports the quantification of small and larger sample peaks in one run. This instrument has standard atmospheric pressure and temperature balance; hence fluctuations in the lab environment do not impact results.
Agilent High performance liquid chromatography (HPLC)
The Agilent 1260 Infinity Quaternary High-Performance Liquid Chromatography (HPLC) system gives students hands-on experience with an important analytical technique used in chemistry, biology, pharmaceutical science, environmental science, and other laboratory-based fields. HPLC is used to separate, identify, and measure individual chemical compounds within a liquid mixture, allowing researchers to determine both what substances are present and their concentrations.
During an analysis, a small liquid sample is introduced into the system and carried by a solvent through a specialized column under high pressure. The compounds within the sample interact differently with the material inside the column, causing them to separate as they move through the system. Detectors then measure the separated compounds and produce data that students can analyze and interpret.
The Agilent 1260 Infinity Quaternary HPLC provides flexibility in solvent selection and supports a wide range of analytical methods. Students gain practical experience with chromatography, instrument operation, data analysis, and laboratory research.
Access to HPLC instrumentation gives students hands-on experience with analytical equipment and techniques used in research, graduate study, and science-related careers.
Perkin Elmer Atomic Absorption Spectroscopy
The PerkinElmer AAnalyst 800 Atomic Absorption Spectrometer gives students hands-on experience measuring the concentration of metals and other elements in a variety of samples. Atomic Absorption Spectroscopy (AAS) can be used to identify and quantify elements such as lead, cadmium, copper, iron, calcium, and sodium, making it an important analytical technique in chemistry, environmental science, and other laboratory fields.
The instrument works by converting a prepared sample into free atoms and passing a specific wavelength of light through them. By measuring how much light the atoms absorb and comparing the results with known standards, the system can determine the concentration of a particular element. The AAnalyst 800 supports sensitive metal analysis and can be used for applications such as testing water and soil, analyzing biological materials, and detecting trace metals and contaminants.
Working with atomic absorption spectroscopy gives students practical experience with sample preparation, analytical instrumentation, calibration, and data analysis—skills used in research, environmental testing, quality control, and other science-related careers.
PerkinElmer NexION® 1000 ICP-MS
The PerkinElmer NexION 1000 Inductively Coupled Plasma Mass Spectrometer (ICP-MS) gives students hands-on experience detecting and measuring extremely small amounts of metals and other elements in a variety of samples. The instrument can analyze multiple elements at very low concentrations, making it particularly valuable for identifying trace elements and potential contaminants.
During analysis, a prepared sample is introduced into a high-temperature argon plasma, where its components are converted into charged particles called ions. The instrument separates these ions according to their mass and measures them to determine which elements are present and their concentrations. Its sensitive detection capabilities allow students and researchers to analyze elements at parts-per-billion and, for some applications, parts-per-trillion levels.
ICP-MS is used for applications such as detecting heavy metals in water, analyzing soil and other environmental samples, evaluating pharmaceuticals and consumer products, and conducting scientific research. The NexION 1000 is designed to reduce analytical interference and provide accurate results even when working with complex samples.
Working with ICP-MS gives students practical experience with advanced analytical instrumentation, trace-element analysis, sample preparation, and data interpretation—skills applicable to research, environmental testing, graduate study, and science-related careers.
Agilent Gas Chromatograph GC 7890
The Agilent 7890 Series Gas Chromatograph (GC) gives students hands-on experience separating and analyzing chemical compounds in complex samples. Gas chromatography is commonly used to determine the components of a mixture, measure their concentrations, and evaluate the purity of a substance. The technique is particularly useful for compounds that can be vaporized without decomposition.
During an analysis, a sample is injected into the instrument and vaporized. A carrier gas moves the sample through a specialized column inside a temperature-controlled oven. Because individual compounds travel through the column at different rates, they separate before reaching a detector, which measures the compounds and produces data that students can analyze and interpret.
The Agilent 7890 provides precise control of temperature, gas pressure, and flow to produce reliable and repeatable results. Gas chromatography has applications in environmental monitoring, pharmaceutical and chemical testing, quality control, and scientific research.
Working with gas chromatography gives students practical experience with analytical instrumentation, sample preparation, and data analysis—skills applicable to research, graduate study, and science-related careers.
Milestone ETHOS UP Microwave Digestion System MTCSC
The ETHOS UP microwave is a powerful digestor with infrared sensors. This closed system also houses contactless temperature sensors for digestion control. Digestion is also enhanced by inbuilt two 950W magnetrons and a diffuser. The latter helps evenly distribute microwaves throughout the chamber. ETHOS UP allows for real-time monitoring of the digestion protocol using a digital camera interface and a smart screen display.
This microwave can be used for a wide range of samples and applications – biologicals [blood samples, tissues, metabolic wastes, etc.], geology [sand, clay, iron ore, soda lime, etc.], environmental analyses [dust, pollen, sludge, river sediments, etc.], food and agriculture [fruits, sugars, roots, plant tissues, etc.], petrochemistry [oils, coal, coke, etc.], cleaning and pharmaceutics [detergents, antibiotics, sugars, lipids, and waxes, etc.].
Thermo Scientific Nicolet™ iS20 FTIR Spectrometer
The Thermo Scientific Nicolet iS20 Fourier Transform Infrared (FTIR) Spectrometer gives students hands-on experience identifying and characterizing chemical substances and materials. FTIR spectroscopy uses infrared light to examine the chemical properties of a sample and can be used to analyze organic compounds, polymers, and some inorganic materials.
During analysis, a sample is exposed to infrared light. Different chemical bonds absorb specific wavelengths of the light, producing a distinctive spectrum that can help identify the material and provide information about its chemical composition. The Nicolet iS20 can analyze a broad range of samples with minimal sample preparation, making FTIR a versatile technique for laboratory analysis.
FTIR spectroscopy is used in fields such as pharmaceuticals, food science, forensics, polymer science, materials analysis, and quality control. Working with the Nicolet iS20 gives students practical experience with spectroscopy, chemical identification, and interpreting analytical data—skills applicable to scientific research, graduate study, and laboratory careers.
Kodak Imaging System
A gel imager is an equipment used in many biochemistry and molecular biology laboratories. It is used to images of stained agarose or polyacrylamide gels. Agarose and polyacrylamide gels are typically used to separate nucleic acids and proteins, respectively. Agarose gels can be stained with fluorescent compounds like ethidium bromide, GelRed, GelBright, etc., and polyacrylamide gels with Coomassie stain, silver, fluorescent dyes like SYPRO orange.
The KODAK Gel Logic 1500 is an advanced imaging system for chemiluminescence, fluorescence, other chromogenic gels, blots, plates, etc. This system is very sensitive, with an integrated 1.4 million pixel cooled CCD camera with mega pixel resolution, a wide sample loading chamber with ultra transluminescence lighting and data analysis software. the enhanced sensitivity is attributed to the cooled camera at -25 degrees Celsius from room temperature to increase signal to noise ratio.
It is applicable for broadband UV detection, nucleic acid gels, protein gels, colorimetric and chemiluminescence analyses. It can be used to excite a variety of dyes using its different illumination modes; Epi UV, Epi white, white-light transillumination, UV transillumination.
Savant Speedvac Plus SC110A Centrifuge
The Savant SC110A Plus SpeedVac is a centrifugal vacuum concentrator used to prepare laboratory samples for analysis. It rapidly removes liquid from samples, allowing dissolved materials to be concentrated or completely dried while helping protect sensitive compounds from excessive heat.
The SpeedVac combines centrifugal force, vacuum pressure, and controlled heat to accelerate evaporation. As samples spin, the vacuum lowers the boiling point of the solvent so liquids can evaporate at relatively low temperatures. Centrifugal force helps keep the samples contained in their tubes, reducing the risk of splashing, foaming, or sample loss during the process.
Students can use the SpeedVac to prepare and concentrate a variety of biological and chemical samples, including DNA and RNA preparations, proteins and peptides, and chromatography fractions. It can also be used to prepare samples for further analysis using techniques such as HPLC and GC/MS.
Working with the SpeedVac gives students practical experience with laboratory sample preparation and handling techniques that are commonly used in scientific research and analytical laboratories.
Agilent Cary Eclipse Fluorescence Spectrometer with micro well plate reader
The Agilent Cary Eclipse Fluorescence Spectrophotometer gives students hands-on experience measuring the light emitted by fluorescent substances. Fluorescence spectroscopy is a highly sensitive analytical technique used to study compounds that emit light after absorbing energy and can provide information about their concentration, structure, and chemical environment.
During analysis, a sample is exposed to a selected wavelength of light, causing certain molecules to become excited and emit light at a different wavelength. The instrument measures this emitted light to help students characterize substances and study chemical or biological processes. With its microplate reader accessory, the Cary Eclipse can automatically analyze many samples in 96- or 384-well plates, making it useful for experiments involving large numbers of samples.
The Cary Eclipse supports fluorescence, phosphorescence, bioluminescence, and chemiluminescence measurements. Applications include studying proteins and other biological molecules, monitoring chemical and biochemical reactions, analyzing fluorescent compounds, and screening multiple samples.
Working with fluorescence spectroscopy gives students practical experience with sensitive analytical measurements, instrument operation, and data interpretation—skills applicable to chemistry, biotechnology, biological research, and other laboratory-based careers.
Bruker Nuclear Magnetic Resonance
It exploits the change in energy levels after exposure to a strong magnetic field, forms the basis of some powerful analytical research tools. Magnetic resonance spectroscopy (MRS) encompasses a variety of related techniques, such as nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) that have a diverse array of research, clinical and industry applications. The non-invasive, non-destructive nature of the technology allows the study of living cells and tissues as well as detailed analysis of solutions and body fluids. A combination of magnetic resonance analyses can provide both functional information and anatomical information, allowing relationships between the two to be determined.
The Nuclear Magnetic Resonance Spectrometer (NMR) consists of a 9.4 Tesla ultrashielded magnet with a 5 mm bore equipped with a broadband tunable probe. The instrument is a state of the art Bruker 400 MHz Avance Neo spectrometer. The instrument is essential for structural elucidation of molecules, monitoring reactions and quantification of reaction products.
Ultrasonic Cell Sonicator 1204P16
The sonicator uses ultrasonic waves to homogenize, lyse or disrupt particles in suspension. It has a full range amplitude control for efficient sample processing and a touch screen interface to load and sync different sequences and sonication methods. Its adjustable pulse mode and temperature monitoring capabilities make it suitable to regulate and monitor temperature for temperature-sensitive samples as well as preventing overheating of the instrument.
It has a variety of applications including extracting pigments, proteins, nuclear material, and other cell organelles from plants, algae, bacteria, yeast, heart, skin tissue, etc. The intensity of the sonication process is regulated based on the cell/tissue type under investigation. Its autotune capacity serves to monitor changes in the ultrasonic frequencies and adjusts them, for a consistent output. Thus a manual autotune is not required.
