Denaturation of Lysozyme

The Paper is here:  Free Energy Changes in Lysozyme Denaturation


The bare bones of the work

1. You want to have concentrations of from 0 to 7 molar Guanadinum Chloride.
• half molar steps from 0-3 M 0.33 M steps from 3-5 half molar steps from 5-7 M
  
2. you  want an absorbsion of about 0.5 OD for the lysozyme.  you have avalable 10 mg/ml lysozyme more or less
• you will need to put this into the buffer listed in the materials section of the paper PBS might also work thats what the protein is
  currently stored in. 
  
3. You need 3 ml of sample for each point
   
4. You should build a table to show the volume of solution you are putting into the test tubes
   
5. we have 3 specs to do this work, on upstairs and two downstairs maybe a fouth.  and 5 teams all of you should put your solutions together on Thursday some of you will be able to do your samples on Thursday and read them that day some of you can read them at other times.  You probably dont want them to sit for more than 2 Days.   If you need  me to I can set up sign up sheets.  
   
6. incubate samples for at least 30 min to reach equilibrium. 
   
7. Data treatment will be discussed in class.